PCR Ready DNA in Minutes
Akonni TruTip uses a patented, innovative nucleic acid binding matrix inserted in a pipette tip. TruTips are available in several formats tailored to the end-user’s throughput needs. Lower throughput samples may be processed using a single or multi-channel electronic pipette. Typical extractions are performed in less than 7 minutes, or 1 minute per extraction with an 8-channel pipette.
TruTip on-demand solutions require no major capital equipment, service contracts, labor intensive processes or hazardous organic solvents. TruTip on-demand solutions are also great for field-based applications, when rapid extractions are required in a “no-electricity”, field setting and without the support of a mobile lab.
Extensive testing on the extraction and purification of genomic DNA (gDNA) from saliva has demonstrated highly reproducible yields at concentrations equivalent to those produced by industry-leading systems. Starting with the Oragene® DNA Collection Kits, saliva can be collected and stored at room temperature, providing a simple and inexpensive sample to procss with Akonni’s TruTips.
Extracted gDNA was detected by real-time PCR on the Roche LightCycler® 480, using the Applied Biosystem™ (AB) Quantifiler® Human DNA Quantification Kit. Concentration was calculated based on AB Human DNA Quantifier Standard Curve analysis.
| 100 / 100 | 2mL SPT TruTip™ | 25.42 ± 0.06 | 21.97 ± 0.99 | 2.2 |
| Qiagen® Blood or Body Fluid |
26.03 ± 0.12 | 13.65 ± 1.12 | 1.37 | |
| 50 / 50 | 1mL SPT TruTip™ | 25.48 ± 0.11 | 21.18 ± 1.69 | 1.06 |
| Qiagen® Blood or Body Fluid |
26.65 ± 0.27 | 8.75 ± 1.89 | 0.44 |
Table 1: Comparison of Average Ct, concentration and yield between Akonni TruTips and Qiagen columns.
Figure 1: Representative fluorescence curves from real-time PCR.
A comparison of the TruTip gDNA Kit with 1 mL and 2 mL SPT tips, against the Qiagen® QlAamp® DNA Mini Kit (Blood or Body Fluid Protocol) was performed (n=8 samples ea. for the 1 mL comparison and n=10 samples for the 2 mL comparison). The same amount of input sample and elution volume was used for direct comparison.
