PCR Ready DNA in Minutes
Akonni TruTip uses a patented, innovative nucleic acid binding matrix inserted in a pipette tip. TruTips are available in several formats tailored to the end-user’s throughput needs. Lower throughput samples may be processed using a single or multi-channel electronic pipette. Typical extractions are performed in less than 7 minutes, or 1 minute per extraction with an 8-channel pipette.
TruTip on-demand solutions require no major capital equipment, service contracts, labor intensive processes or hazardous organic solvents. TruTip on-demand solutions are also great for field-based applications, when rapid extractions are required in a “no-electricity”, field setting and without the support of a mobile lab.
Extensive testing on the extraction and purification of genomic DNA (gDNA) from saliva has demonstrated highly reproducible yields at concentrations equivalent to those produced by industry-leading systems. Starting with the Oragene® DNA Collection Kits, saliva can be collected and stored at room temperature, providing a simple and inexpensive sample to procss with Akonni’s TruTips.
Extracted gDNA was detected by real-time PCR on the Roche LightCycler® 480, using the Applied Biosystem™ (AB) Quantifiler® Human DNA Quantification Kit. Concentration was calculated based on AB Human DNA Quantifier Standard Curve analysis.
|100 / 100||2mL SPT TruTip™||25.42 ± 0.06||21.97 ± 0.99||2.2|
|Qiagen® Blood or
|26.03 ± 0.12||13.65 ± 1.12||1.37|
|50 / 50||1mL SPT TruTip™||25.48 ± 0.11||21.18 ± 1.69||1.06|
|Qiagen® Blood or
|26.65 ± 0.27||8.75 ± 1.89||0.44|
Table 1: Comparison of Average Ct, concentration and yield between Akonni TruTips and Qiagen columns.
Figure 1: Representative fluorescence curves from real-time PCR.
A comparison of the TruTip gDNA Kit with 1 mL and 2 mL SPT tips, against the Qiagen® QlAamp® DNA Mini Kit (Blood or Body Fluid Protocol) was performed (n=8 samples ea. for the 1 mL comparison and n=10 samples for the 2 mL comparison). The same amount of input sample and elution volume was used for direct comparison.