An Array of Diagnostic Information at Your Fingertips

Manufacturing

TruArrays™ are manufactured by co-polymerization on a glass or plastic substrate. Printing and flow cell assembly occur in a class 10,000 temperature- and humidity-controlled cleanroom. Oligonucleotide probes are synthesized in-house via standard phosphoramidite chemistry and HPLC purified. Stock oligonucleotide solutions are bar-coded and stored at -20°C in an automated REMP® sample storage system. Prior to a print run, the oligonucleotide probes are combined with pre-polymer solution in a 384-well source plate using custom TECAN® robotics. Pre-polymer solution is applied to the array glass substrate using Genetix QArray printers and custom blunt pins. After printing, arrays are photopolymerized with UV light, washed to remove unbound probe, and air dried. Flow cells are assembled via a laser-cut gasket and optically clear top-plate that contains an inlet port, the microarray chamber (~25 µL), a waste chamber (~1 mL) and a vented outlet port. Approximately 10% of a microarray production run (batch) undergoes quality control checks, including visual and spectrophotometric inspections of spot number, size and morphology. Product barcodes are applied to the device, followed by packaging in vacuum sealed wrappers or microscope slide boxes.

Figure A: REMP SSS and TECAN automated source plate system. Figure B: Genetix QArray printers in class 10,000 clean room. Figure C: Depiction of the single-step printing and co-polymerization.